Just a quick update on this, I had a good E mail exchange with AdGuard support and they confirmed the primary WAN DNS servers should be set to their public DNS servers, rather than the default ISP DNS, and they then advised how to set everything up manually (rather than via the toggle switch) for DNS over TLS.
They also got me to set up my DDNS details within the device (router) settings within AdGuard.
It is likely the problem I was experiencing was caused by a change in my public IP / WAN address.
I made the changes on Sunday afternoon and so far so good.
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